Characterization of Cultivar Differences of Blueberry Wines Using GC-QTOF-MS and Metabolic Profiling Methods

摘要

A non-targeted volatile metabolomic approach based on the gas chromatography-quadrupoletime of fight-mass spectrometry (GC-QTOF-MS) coupled with two different sample extraction techniques(solid phase extraction and solid phase microextraction) was developed. Combined mass spectra ofblueberry wine samples, which originated from two different cultivars, were subjected to orthogonalpartial least squares-discriminant analysis (OPLS-DA). Principal component analysis (PCA) revealsan excellent separation and OPLS-DA highlight metabolic features responsible for the separation.Metabolic features responsible for the observed separation were tentatively assigned to phenylethylalcohol, cinnamyl alcohol, benzenepropanol, 3-hydroxy-benzenethanol, methyl eugenol, methylisoeugenol, (E)-asarone, (Z)-asarone, and terpenes. Several of the selected markers enabled adistinction in secondary metabolism to be drawn between two blueberry cultivars. It highlights themetabolomic approaches to find out the influence of blueberry cultivar on a volatile compositionin a complex blueberry wine matrix. The distinction in secondary metabolism indicated a possibleO-methyltransferases activity difference among the two cultivars.