A Novel Yeast Mutation, rad52-L89F, Causes a Specific Defect in Rad51-Independent Recombination That Correlates With a Reduced Ability of Rad52-L89F to Interact With Rad59

摘要

We isolated a novel rad52 mutation, rad52-L89F , which specifically impairs recombination in rad51 Δ cells. rad52-L89F displays phenotypes similar to rad59 Δ and encodes a mutant protein impaired in its ability to interact with Rad59. These results support the idea that Rad59 acts in homologous recombination via physical interaction with Rad52.