A Novel Yeast Mutation rad52-L89F Causes a Specific Defect in Rad51-Independent Recombination That Correlates With a Reduced Ability of Rad52-L89F to Interact With Rad59

摘要

We isolated a novel rad52 mutation, rad52-L89F, which specifically impairs recombination in rad51Δ cells. rad52-L89F displays phenotypes similar to rad59Δ and encodes a mutant protein impaired in its ability to interact with Rad59. These results support the idea that Rad59 acts in homologous recombination via physical interaction with Rad52.