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首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >Analysis of the Proteins Involved in the in Vivo Repair of Base–Base Mismatches and Four-Base Loops Formed During Meiotic Recombination in the Yeast Saccharomyces cerevisiae
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Analysis of the Proteins Involved in the in Vivo Repair of Base–Base Mismatches and Four-Base Loops Formed During Meiotic Recombination in the Yeast Saccharomyces cerevisiae

机译:酵母酿酒酵母减数分裂重组过程中形成的碱基-碱基错配和四碱基环的体内修复中涉及的蛋白质分析

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DNA mismatches are generated when heteroduplexes formed during recombination involve DNA strands that are not completely complementary. We used tetrad analysis in Saccharomyces cerevisiae to examine the meiotic repair of a base–base mismatch and a four-base loop in a wild-type strain and in strains with mutations in genes implicated in DNA mismatch repair. Efficient repair of the base–base mismatch required Msh2p, Msh6p, Mlh1p, and Pms1p, but not Msh3p, Msh4p, Msh5p, Mlh2p, Mlh3p, Exo1p, Rad1p, Rad27p, or the DNA proofreading exonuclease of DNA polymerase δ. Efficient repair of the four-base loop required Msh2p, Msh3p, Mlh1p, and Pms1p, but not Msh4p, Msh5p, Msh6p, Mlh2p, Mlh3p, Exo1p, Rad1p, Rad27p, or the proofreading exonuclease of DNA polymerase δ. We find evidence that a novel Mlh1p-independent complex competes with an Mlhp-dependent complex for the repair of a four-base loop; repair of the four-base loop was affected by loss of the Mlh3p, and the repair defect of the mlh1 and pms1 strains was significantly smaller than that observed in the msh2 strain. We also found that the frequency and position of local double-strand DNA breaks affect the ratio of mismatch repair events that lead to gene conversion vs . restoration of Mendelian segregation.
机译:当重组过程中形成的异源双链体涉及不完全互补的DNA链时,就会产生DNA错配。我们在酿酒酵母中使用了四联体分析,以检验野生型菌株以及与DNA错配修复相关的基因突变的菌株的碱基-碱基错配和四碱基环的减数分裂修复。有效修复碱基错配需要Msh2p,Msh6p,Mlh1p和Pms1p​​,但不需要Msh3p,Msh4p,Msh5p,Mlh2p,Mlh3p,Exo1p,Rad1p,Rad27p或DNA聚合酶δ的DNA校对核酸外切酶。要有效修复四碱基环,需要Msh2p,Msh3p,Mlh1p和Pms1p​​,但不需要Msh4p,Msh5p,Msh6p,Mlh2p,Mlh3p,Exo1p,Rad1p,Rad27p或DNA聚合酶δ的校对核酸外切酶。我们发现新的Mlh1p独立复合物与Mlhp依赖复合物竞争四碱基环修复的证据。四碱基环的修复受到Mlh3p缺失的影响,而mlh1和pms1菌株的修复缺陷明显小于msh2菌株中的修复缺陷。我们还发现,局部双链DNA断裂的频率和位置会影响导致基因转换vs.的错配修复事件的比率。恢复孟德尔种族隔离。

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