Blood Meal Analysis to Identify Reservoir Hosts for Amblyomma americanum Ticks

摘要

Efforts to identify wildlife reservoirs for tick-borne patho-gens are frequently limited by poor understanding of tick–host interactions and potentially transient infectivity of hosts under natural conditions. To identify reservoir hosts for lone star tick (Amblyomma americanum)–associated pathogens, we used a novel technology. In fi eld-collected ticks, we used PCR to amplify a portion of the 18S rRNA gene in rem-nant blood meal DNA. Reverse line blot hybridization with host-specifi c probes was then used to subsequently detect and identify amplifi ed DNA. Although several other taxa of wildlife hosts contribute to tick infection rates, our results confi rm that the white-tailed deer (Odocoileus virginianus) is a reservoir host for several A. americanum–associated pathogens. Identifi cation of host blood meal frequency and reservoir competence can help in determining human infec-tion rates caused by these pathogens