编码美洲螯龙虾肽聚糖识别蛋白(PGRP)cDNA的电子克隆及其生物信息学分析

摘要

We obtained a cDNA sequence that encoding a putative peptidoglycan recognition protein (PGRP) of American lobster,Homarus americanus,by in silicon cloning in this study; and analyzed the deduced protein by bioinformatics methods in terms of its general physical and chemical properties,hydrophobicity,signal peptide,secondary / tertiary structure and localization sites in cells.The results show that the obtained 920 bp long cDNA contains an open reading frame (834 bp) which encoding a protein with 277 amino acids.The protein has a signal peptide in its N terminal,a PGRP domain in its C terminal,which means that the protein maybe is a secretory protein,short type PGRP.Phylogeny analysis indicates that the protein is more like the PGRP-SC2 and PGRP-SD in Drosophila melanogaster.%利用电子克隆方法,获得编码美洲螯龙虾(Homarus americanus)肽聚糖识别蛋白(peptidoglycan recognition protein,PGRP)的cDNA序列及相应的氨基酸序列；采用生物信息学方法对其进行基本理化性质、亲疏水性、信号肽、二级结构、三级结构、细胞定位等的分析.结果获得一段长920 bp的编码美洲螯龙虾PGRP的cDNA序列,包含一个长834 bp的开放阅读框,编码277个氨基酸；编码蛋白是含有信号肽的分泌蛋白,含有1个C端PGRP结构域,表明是一种PGRP;分析结果显示该蛋白与黑腹果蝇(Drosophila melanogaster)短型PGRP-SC2和PGRP-SD比较相似.