Adenovirus Type 7 Peptide Diversity during Outbreak, Korea, 1995–2000

摘要

To understand the molecular basis of observed region-al shifts in the genome types of adenovirus type 7 (Ad7)isolated in Korea during nationwide outbreaks from 1995 to2000, the genetic variabilities of Ad7d and Ad7l were stud-ied by sequence analysis of hexon, fiber, E3, and E4 openreading frame (ORF) 6/7 peptides. One amino acid changein the receptor-binding domain of fiber and 6 amino acidvariations in E4 ORF 6/7 were identified between 2genome types, while no variations were found in hexon andE3. Phylogenetic trees based on hexon, fiber, and E4 sug-gested that the Ad7 epidemic was probably caused by theintroduction of the Japanese Ad7d strains. Our data alsoprovide evidence that the rapid divergence of Ad7d to anovel genome type Ad7l could have been due to viralstrategies involving multiple sequence changes in E4. Thisresult suggests fiber and E4 ORF 6/7 peptides participatein the evolution of Ad7