Antimicrobial Efficacy of New Investigational Multipurpose Disinfecting Solution and Comparison to Commercially Available Multipurpose Disinfecting Solutions

摘要

Purpose: To evaluate antimicrobial efficacy of investigational multipurpose formulation against bacteria, fungi and Acanthamoeba spp. and compare it to commercially available multipurpose disinfecting solutions (MPS). Study was done according to ISO 14729:2001/A.2010 standard. Methods: The multipurpose disinfecting solutions studied were - Investigational MPS-1: polyhexamethylene biguanide (PHMB) + poloxamer (PLX) and currently marketed Japan products MPS-2: polyquaternium (PQ1) + tetronic 1304, MPS-3: PHMB + poloxamine (PLA) and MPS-4: PHMB +PLX. Test organisms were: Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 9027), Serratia marcescens (ATCC 13880), Candida albicans (ATCC 10231), Fusarium solani (ATCC 36031) and Gram-negative clinical isolates. Test solutions were evaluated at the minimum recommended disinfection time of 4 hours. Disinfectant efficacy of MPS was also evaluated against Acanthamoeba trophozoites. Results: After 4 hours exposure, Investigational MPS-1 and MPS-3 showed 3 log kill of ISO bacteria and fungi. MPS-2 and MPS-4 failed to meet stand-alone criteria for ISO bacteria and fungi. MPS-1, MPS-3 and MPS-4 showed 3 log kill against clinical isolates, while MPS-2 showed 1 log kill. MPS-1 and MPS-3 achieved 3 log reduction for Acanthamoeba trophozoites, while MPS-4 achieved 2 log reduction and MPS-2 achieved 1 log reduction. Conclusions: New Investigational MPS-1 exhibited broad antimicrobial activity against ISO panel organisms and showed additional robustness against clinical isolates and Acanthamoeba trophozoites. MPS-1 showed similar activity as MPS-3, while MPS-2 and MPS-4 failed to meet stand-alone criteria against ISO organisms and clinical isolates.