Comparison of methodological approaches for detection of major rifampin and izoniazid resistance conferring mutations in Mycobacterium tuberculosis

摘要

Background: Emergence of multidrug-resistant Mycobacterium tuberculosis strains and their global dissemination necessitate development, evaluation and comparison of the rapid molecular tests that target genetic determinants of bacterial drug resistance. A wide range of such methods is available at present and the choice of those most appropriate is among the pertinent task of the National Tuberculosis Control Program. The objective of this study was to compare different molecular methods for detection of the major rifampin (RIF) and isoniazid (INH) resistance conferring mutations in M.