Phylogenetic relationships of Acanthamoeba spp. based on multiple gene analysis

摘要

Background: The detection of Acanthamoeba using morphological analysis is comparatively easier, but the taxonomy at the species level still remains unclear. Many researchers have been used various kinds of non-morphological methods for the taxonomy of Acanthamoeba spp. as several studies have been demonstrated that there are numerous inconsistencies between the classification of Acanthamoeba strains. To evaluate the genetic diversity we have analyzed firstly nuclear small subunit ribosomal RNA (18S rRNA) gene and now we are reexamining by using mitochondrial small subunit ribosomal RNA (Mt 16S rRNA) gene.