Development of a reverse-transcription isothermal amplification assay for rapid detection and genotyping of Hepatitis C virus infection in blood

摘要

Background: Hepatitis C virus (HCV) an important blood-borne pathogen and due to its silent chronicity, may go undetected in infected individuals for decades. In the United States and in many developed countries, blood specimens are currently screened for the presence of HCV antibodies and nucleic acids, rendering blood supplies as very safe. However, the global prevalence of HCV infection highlights the urgent need for new testing tools that are suitable for broader use including by underprivileged countries.