Correction for Wertz et al., “Genomic and Physiological Characterization of the Verrucomicrobia Isolate Didymococcus colitermitum gen. nov., sp. nov., Reveals Microaerophily and Nitrogen Fixation Genes”

摘要

Description of Didymococcus colitermitum sp. nov. Didymococcus colitermitum sp. nov. (co.li.ter'mi.tum. L. n. colon, colon, part of the large intestine; L. n. termes -itis, wood-eating worm, termite; N.L. gen. pl. n. colitermitum, of the gut of termites). Cells are coccoid (0.25 μm to 0.5 μm in diameter) and occur almost exclusively in pairs, with a Gram-negative cell wall morphology that includes an outer membrane. Cells are nonmotile, obligate aerobes and are microaerophilic. The shortest generation times occur in liquid medium under an atmosphere of 2 to 8% O2 (balance N2). On solid R2A medium, colonies are 2 to 4 mm in diameter, have an entire margin and a low convex, mucoid morphology, and are cream colored. Cells do not possess catalase or NADH/NADPH peroxidase activity. Nitrogenase activity is inferred through growth on nitrogen-free medium. Growth occurs in liquid media between 15 and 35°C (optimum, 30°C); there is no growth at 37°C or 4°C. Growth occurs at a pH range of 5.5 to 7.5 (optimum, 7.0); there is no growth at a pH of ≤5 or ≥8. Substrates utilized as energy sources include starch, d-cellobiose, d-maltose, d-glucose, d-galactose, and one or more components present in yeast extract. Microcrystalline cellulose, methylcellulose, carboxy methylcellulose, xylan, d-fructose, d-mannose, d-trehalose, sucrose, d-ribose, d-xylose, l-arabinose, d-mannitol, d-sorbitol, d-raffinose, dl-lactate, sodium pyruvate, sodium fumarate, sodium acetate, allantoin, d-glucuronate, d-galacturonate, d-gluconic acid, xanthine, tannic acid, resourcinol, vanillic acid, sodium benzoate, and trimethylbenzoate are not utilized. The genome of type strain TAV2 is 5.2 Mb in size, contains 60.5 mol% G+C, and possesses one 16S rRNA gene copy. The type strain, isolated from guts of Reticulitermes flavipes (Kollar) collected in Dansville, MI, is TAV2 (ATCC BAA-2264; DSM 25453).