New Insights into the Genetic Organization of the FK228 Biosynthetic Gene Cluster in Chromobacterium violaceum No. 968

摘要

The biosynthetic gene cluster of FK228, an FDA-approved anticancer natural product, was identified and sequenced previously. The genetic organization of this gene cluster has now been delineated through systematic gene deletion and transcriptional analysis. As a result, the gene cluster is redefined to contain 12 genes: depA through depJ , depM , and a newly identified pathway regulatory gene, depR .