Chemoenzymatic Synthesis of N-Acetyl-d-Neuraminic Acid from N-Acetyl-d-Glucosamine by Using the Spore Surface-Displayed N-Acetyl-d-Neuraminic Acid Aldolase

摘要

Chemoenzymatic synthesis of N -acetyl-d-neuraminic acid from N -acetyl-d-glucosamine using the spore surface-displayed N -acetyl-d-neuraminic acid aldolase at a high concentration (53.9 g liter~(?1)) was achieved in this study. Thus, displaying a target enzyme on the surface of spores might be an alternative for integration of biocatalytic conversion into chemical synthesis.