Moonlighting Role of a Poly-γ-Glutamate Synthetase Component from Bacillus subtilis: Insight into Novel Extrachromosomal DNA Maintenance

摘要

The Bacillus subtilis structural gene pgsE was investigated as a tool for extrachromosomal DNA maintenance (EDM). It ameliorated the stability of high-copy-number vectors, regardless of whether they were derived from rolling-cycle or theta-mode replicons, without any selective pressure. This unique EDM phenomenon may occur via a trans -acting mechanism.