A mechanical cell disintegrator is described which efficiently ruptures bacteria, molds, actinomycetes, and yeasts. Cells were broken continuously in a chamber by rapid shaking of a mixture of cell suspension, plastic beads, and air. Volume capacity for complete breakage varied with experimental conditions and the microorganism involved, and ranged from 840 ml/hr to greater than 4,470 ml/hr. Subcellular preparations made with the machine retained a high level of enzymatic activity.
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机译：描述了一种机械细胞崩解剂，其有效地使细菌，霉菌，放线菌和酵母破裂。通过快速摇动细胞悬液，塑料珠和空气的混合物，在室内连续破碎细胞。完全破损的体积容量随实验条件和所涉及的微生物而变化，范围为840 ml / hr至大于4,470 ml / hr。用该机器制成的亚细胞制剂保留了高水平的酶活性。
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