...
首页> 外文期刊>Applied and Environmental Microbiology >Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli.
【24h】

Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli.

机译:通过在大肠杆菌中过表达marA,提高了有机溶剂的耐受性和抗生素抗性。

获取原文

摘要

We previously reported that overexpression of the soxS or robA gene causes in several Escherichia coli strains the acquisition of higher organic solvent tolerance and also increased resistance to a number of antibiotics (H. Nakajima, K. Kobayashi, M. Kobayashi, H. Asako, and R. Aono, Appl. Environ. Microbiol. 61:2302-2307, 1995). Most E. coli strains cannot grow in the presence of cyclohexane. We isolated the marRAB genes from a Kohara lambda phage clone and cyclohexane-tolerant mutant strain OST3408. We found a substitution of serine for arginine at position 73 in the coding region of marR of OST3408 and designated the gene marR08. Our genetic analysis revealed that marR08 is responsible for the cyclohexane-tolerant phenotype. We observed that the marA gene on high-copy-number plasmids increased the organic solvent tolerance of E. coli strains. Furthermore, exposure of E. coli cells to salicylate, which activates the mar regulon genes, also raised organic solvent tolerance. Overexpression of the marA, soxS, or robA gene increased resistance to numerous antibiotics but not to hydrophilic aminoglycosides.
机译:我们先前曾报道过soxS或robA基因的过度表达导致几种大肠杆菌菌株获得更高的有机溶剂耐受性,并且还增加了对多种抗生素的抗性(H. Nakajima,K。Kobayashi,M。Kobayashi,H。Asako,和R.Aono,Appl.Environ.Microbiol.61:2302-2307,1995)。大多数大肠杆菌菌株不能在环己烷存在下生长。我们从Koharaλ噬菌体克隆和耐环己烷突变株OST3408中分离了marRAB基因。我们在OST3408的marR编码区的第73位发现了精氨酸的丝氨酸替代物,并命名为基因marR08。我们的遗传分析表明,marR08负责环己烷耐受的表型。我们观察到高拷贝数质粒上的marA基因增加了大肠杆菌菌株的有机溶剂耐受性。此外,将大肠杆菌细胞暴露于水杨酸盐中,从而激活了mar regulon基因,也提高了有机溶剂的耐受性。 marA,soxS或robA基因的过表达增加了对多种抗生素的抵抗力,但对亲水性氨基糖苷没有抵抗力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号