Seven toluene-oxidizing bacterial strains (Pseudomonas mendocina KR1, Burkholderia cepacia G4, Pseudomonas putida F1, Pseudomonas pickettii PKO1, and Pseudomonas sp. strains ENVPC5, ENVBF1, and ENV113) were tested for their ability to degrade chloroform (CF). The greatest rate of CF oxidation was achieved with strain ENVBF1 (1.9 nmol/min/mg of cell protein). CF also was oxidized by P. mendocina KR1 (0.48 nmol/min/mg of cell protein), strain ENVPC5 (0.49 nmol/min/mg of cell protein), and Escherichia coli DH510B(pRS202), which contained cloned toluene 4-monooxygenase genes from P. mendocina KR1 (0.16 nmol/min/mg of cell protein). Degradation of [14C]CF and ion analysis of culture extracts revealed that CF was mineralized to CO2 (approximately 30 to 57% of the total products), soluble metabolites (approximately 15%), a total carbon fraction irreversibly bound to particulate cellular constituents (approximately 30%), and chloride ions (approximately 75% of the expected yield). CF oxidation by each strain was inhibited in the presence of trichloroethylene, and acetylene significantly inhibited trichloroethylene oxidation by P. mendocina KR1. Differences in the abilities of the CF-oxidizing strains to degrade other halogenated compounds were also identified. CF was not degraded by B. cepacia G4, P. putida F1, P. pickettii PKO1, Pseudomonas sp. strain ENV113, or P. mendocina KRMT, which contains a tmo mutation.
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机译：测试了七种甲苯氧化细菌菌株（门氏假单胞菌KR1，伯克霍尔德氏菌G4，恶臭假单胞菌F1，假单胞菌Pickettii PKO1和假单胞菌sp。菌株ENVPC5，ENVBF1和ENV113）降解氯仿（CF）的能力。 CF氧化率最高的菌株是ENVBF1菌株（1.9 nmol / min / mg细胞蛋白）。 CF也被门氏假单胞菌KR1（0.48 nmol / min / mg细胞蛋白），菌株ENVPC5（0.49 nmol / min / mg细胞蛋白）和大肠杆菌DH510B（pRS202）氧化，其中含有克隆的甲苯4-单加氧酶P. mendocina KR1的基因（0.16 nmol / min / mg细胞蛋白）。 [14C] CF的降解和培养物提取物的离子分析表明，CF矿化成CO2（约占总产品的30％至57％），可溶性代谢物（约15％），总碳部分不可逆地结合到颗粒细胞成分上（约30％）和氯离子（约占预期收率的75％）。在三氯乙烯的存在下，每种菌株的CF氧化均被抑制，而乙炔则通过门氏假单胞菌KR1显着抑制了三氯乙烯的氧化。还确定了CF-氧化菌株降解其他卤代化合物的能力的差异。 CF不会被洋葱伯克霍尔德氏菌G4，恶臭假单胞菌F1，恶臭假单胞菌PKO1，假单胞菌sp。菌株ENV113或P.mendocina KRMT，含有tmo突变。
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