Bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of Treponema hyodysenteriae. Isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and isolates (162) from pigs with dysentery were cultured anaerobically on a rumen fluid-based medium and characterized. Most (71%) of the isolates from colonic epithelia of normal pigs were gram positive, whereas 88% of the epithelia-associated isolates from pigs with dysentery were gram negative. The geometric mean of colony counts was 5.7 X 10(7)/cm2 of colonic tissue from three normal pigs and 7.7 X 10(8)/cm2 from four pigs with dysentery. A number of isolates obtained from contents of the lumens of normal pigs with dysentery were also characterized. Comparison of isolates from epithelial tissue and from contents of the lumens of the same pig indicated that these populations were different. Our results indicate that physiological changes that occur in the colons of pigs with dysentery are accompanied by marked changes in the microbial populations in the colons. The factors which regulate the population changes are not yet understood.
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机译：将断奶的健康猪近端结肠黏膜上的细菌种群与接种猪痢疾密螺旋体培养物引起的痢疾猪种群进行比较。在基于瘤胃液的培养基上厌氧培养代表正常猪结肠上皮的主要菌群的分离株（136）和痢疾猪的分离株（162）。正常猪结肠上皮分离株的大多数（71％）为革兰氏阳性，而痢疾猪的上皮相关分离株的88％为革兰氏阴性。三个正常猪的结肠组织的菌落计数的几何平均值为5.7 X 10（7）/ cm2，四个痢疾猪的结肠组织的菌落计数的几何平均值为7.7 X 10（8）/ cm2。还表征了从患有痢疾的正常猪的内腔内容物中获得的许多分离株。比较同一猪的上皮组织和管腔内的分离物表明，这些种群不同。我们的结果表明，痢疾猪结肠中发生的生理变化伴随着结肠中微生物种群的显着变化。尚不清楚调节人口变化的因素。
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