Validity of a Simple Automated Method in Which the "Direct" Kober Reaction is Used to Measure Estrogens during Pregnancy

摘要

Using a simple automated procedure for measurement of total estrogens in urine, we found that several estrogen conjugates gave higher fluorescence yields than did the corresponding free steroids. In the analysis of urine samples from pregnant women, the "direct Kober reaction" gave consistently higher values than those obtained after preliminary acid or enzymatic hydrolysis. The effects of storage and urine dilution on results for estrogens were also investigated.