Negative Interference by Residual Proteins in the Supernatant Fluid Used in Radioisotopic Assay of Serum Vitamin B12

摘要

Separation of vitamin B12, from serum by heat denaturation results in an individually variable amount of residual protein in the supernatant fluid. I show that during radioisotopic assay of serum vitamin B12, such protein may seriously interfere with the results, and that the interference may be abolished by changing the experimental conditions.