A Rapid Procedure for Catalase Determination in Blood and Tissue Samples with the Van Slyke Manometric Apparatus

摘要

A precise and rapid gasometric procedure is described for catalase determination in blood and tissue samples. The enzyme activity assay is performed with the Van Slyke manometric apparatus. In the developed technicw, a 45-sec. reaction period is used, and a large liquid-gas interphase is provided with the purpose of preventing the oxygen diffusion from becoming a rate-limiting factor. Under the devised methodological conditions, the rate of oxygen evolution closely follows a parabolic curve which permits the calculation of the initial activity velocity of the enzyme. The oxygen evolvement is directly proportional to the quantity of catalase present in the analyzed sample. Less than 4 min. are required for each catalase measurement.