1H‐NMR‐based Metabolomic Study of Miamiensis avidus‐infected Olive Flounder

摘要

The scuticociliate Miamiensis avidus (M. avidus) is an aetiological agent of mass mortalities in cultured fish worldwide. The external signs of infection by this histiophagous parasite are hemorrhage, muscle ulceration, and exophthalmia. In this study, we infected olive flounder (Paralichthys olivaceus) with M. avidus under laboratory conditions, and used 1Ha??nuclear magnetic resonance (NMR) spectroscopy to detect the metabolomic alterations during the infection. Multivariate data analysis showed that there were distinct differences in metabolomes between the control and the infected groups. Analysis of the metabolomic changes indicated that the olive flounder experienced severe metabolic shifts to anaerobic respiration and hypoxia.