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Evaluation of FHIT gene alterations in ovarian cancer

机译:卵巢癌中FHIT基因改变的评估

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The FHIT gene, recently cloned and mapped on chromosome 3p14.2, has frequently been found to be abnormal in several established cancer cell lines and primary tumours. As alterations of chromosome 3p are common events in ovarian cancers with breakpoint sites at 3p14.2, we decided to investigate the role of FHIT in human ovarian tumorigenesis. Fifty-four primary ovarian carcinomas were studied by reverse transcription of FHIT mRNA followed by polymerase chain reaction (PCR) amplification and sequencing of products. The same tumours and matched normal tissues were also investigated for loss of heterozygosity using three microsatellite markers located inside the gene. We found an abnormal transcript of the FHIT gene in two cases (4%) and allelic losses in eight cases (15%). Twelve (22%) of the 54 tumours investigated belonged to young patients with a family history of breast/ovarian cancer. In none of these cases was the FHITgene found to be altered. Our results indicate that FHITplays a role in a small proportion of ovarian carcinomas.
机译:FHIT基因最近被克隆并定位在3p14.2染色体上,在一些已建立的癌细胞系和原发性肿瘤中经常被发现是异常的。由于3p染色体的改变在具有3p14.2断裂点的卵巢癌中很常见,因此我们决定研究FHIT在人类卵巢肿瘤发生中的作用。研究人员通过逆转录FHIT mRNA,然后进行聚合酶链反应(PCR)扩增和产物测序研究了54种原发性卵巢癌。还使用位于基因内部的三个微卫星标记研究了相同的肿瘤和匹配的正常组织的杂合性丧失。我们发现2例(4%)的FHIT基因转录异常,8例(15%)的等位基因缺失。在调查的54种肿瘤中,有十二种(22%)属于具有乳腺癌/卵巢癌家族史的年轻患者。在这些情况下,均未发现FHIT基因发生改变。我们的结果表明FHIT在一小部分卵巢癌中起作用。

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