Study on Solid Phase Extraction and Spectrophotometric Determination of Nickel in Waters and Biological Samples

摘要

A sensitive, selective and rapid method for the determination of nickel based on the rapid reaction of nickel(II) with QADMAA and the solid phase extraction of the Ni(II)-QADMAA chelate with C₁₈ membrane disks has been developed. In the presence of pH 6.0 buffer solution and sodium dodecyl sulfonate (SDS) medium, QADMAA reacts with nickel to form a violet complex of a molar ratio of 1 : 2 (nickel to QADMAA). This chelate was enriched by solid phase extraction with C₁₈ membrane disks. An enrichment factor of 50 was obtained by elution of the chelates form the disks with the minimal amount of isopentyl alcohol. The molar absorptivity of the chelate was 1.32 】 105 L mol-1 cm-1 at 590 nm in the measured solution. Beer`s law was obeyed in the range of 0.01-0.6 レg/mL. This method was applied to the determination of nickel in water and biological samples with good results.