Effect of Glycerophospholipid on Oleate-Dependent Phospholipase D in Mammalian Source

摘要

Effects of exogenous glycerophospholipids on oleate-dependent phospholipase D (PLD) activity were studied in lymphocytic mouse leukemia L1210 cells and in solubilized microsomal phospholipase D of rat brain. Among the phospholipids tested phosphatidic acid had the most stimulatory effects on both PLD activities up to about 3 folds. Lysophosphatidic acid also showed promoting effect on microsomal PLD activity but much less on L1210 cells compared to that of phosphatidic acid. While phosphatidylethanolamine increased PLD activity slightly, phosphatidylinositides were nearly ineffective in the tested sources. The stimulatory effect of phosphatidic acid observed can be utilized to improve the in vitro assay system for oleate-dependent PLD in mammalian sources.