The Zoonotic Risk ofAncylostoma ceylanicumIsolated from Stray Dogs and Cats in Guangzhou, South China

摘要

Canine and feline hookworm infection is endemic in many countries with zoonotic transmission representing a potentially significant public health concern. However, there is limited data available on the zoonotic transmission of canine and feline hookworms in China. This study was conducted to evaluate the zoonotic risk ofAncylostoma ceylanicumisolated from stray dogs and cats in Guangzhou, south China. Primer pairs CAF/CAR were designed to amplify complete ITS sequences of obtainedA. ceylanicum. The results were compared with fourteen ITS reference sequences of human-derivedA. ceylanicumregistered in GenBank, and phylogenetic trees were established by using NJ and ML methods. The sequence similarity of three dog-derived and five cat-derivedA. ceylanicumwith fourteen human-derivedA. ceylanicumwere 96.8%~100% and 97.8%~100%, respectively. Phylogenetic analysis placedA. ceylanicumisolated from dogs and cats in the same group withA. ceylanicumhuman isolates. Due to the ability ofA. ceylanicumto cause a patent infection in humans, the zoonotic risk arising from dog and cat reservoirs to communities in this region should be determined.