DETECTION OF QUINOLONE-RESISTANCE MUTATIONS OF PARC GENE IN CLINICAL ISOLATES OF ACINETOBACTER BAUMANNII IN IRAN (SHORT COMMUNICATION)

摘要

Background: The purpose of this study was to screen of parC gene mutations in clinical isolates of Acinetobacter baumannii from intensive care units (ICUs) of Alzahra Hospital, Isfahan, Iran.Materials and Methods: Seventy isolates of A. baumannii between March 2011 and June 2012 were studied. Susceptibility test was established by E-test method. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing was performed for detection of parC gene mutation.Results: 77.1% of specimens were highly resistant. Mutation at position 80 in parC was observed in 93% of isolates.Conclusion: High proportion of A. baumannii isolates had a mutation in parC that can play an important role in increased incidence of these isolates.