????? ????? ?????? ??? Real-Time PCR ?? ??????? ?? ?? ?????? ??? ????? ????????? ? ??????? ???

??????? ???; ????????? ????; ??????? ????; ????????? ????

首页> 外文期刊>Jundishapur Scientific Medical Journal >????? ????? ?????? ??? Real-Time PCR ?? ??????? ?? ?? ?????? ??? ????? ????????? ? ??????? ???

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????? ????? ?????? ??? Real-Time PCR ?? ??????? ?? ?? ?????? ??? ????? ????????? ? ??????? ???

机译：?????? ?????? ?????? ???实时PCR ?? ??????? ?? ?? ?????? ??? ?????? ????????? ？ ??????? ???

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????? ? ???: ?????? ????mRNA ??? ??????? ?? Real-Time PCR ???-????? ??? ????????? ??-??? ????? ?? ??????? ???????? ??????? ?????? ???? ???? ???. ??? ??? ????? ?????? ??????PCR ??????? ??? ?? ??????? ?? ?? ??? ??? ?? ?????? ????????? ? ???-?????LinRegPCR ???? ????? ???????? ???? ???? ?????? ?????? PCR ??-????.??? ?????: ?? ??? ????? ?? ?? ???-???? ? ??????? RNA ? ???? cDNA?????? ?qRT-PCR ????? ??.??? ?????? PCR Efficiency ?? ?? ??? ??? ????? ????????? ? ?????? ?? ??????? ?? ???-?????LinReg PCR ????? ???? ? ?? ????? ??? ?? ??? ?????? ?? ?? ?????? ????.?????-??: ????? ?????? PCR ?????? ??? ???? ?? ??GAPDH? TGF β? IL-10 ?? ??? ????? ????????? ??-????? 99/1? 81/1 ? 87/1 ? ?????? ?? ??? ??????? ?? ???-????? ??????-?? LinReg PCR ?????? ??? ?? ?? ??-????? 98/1? 82/1 ?82/1 ??-??? ???.?????-????: ????? ??? ????? ???? ??-??? ?? ?? ?????? ????-??? ???? ?? ????? cDNA ????? ??????PCR ???-??? ???? ???? ?? ?? GAPDH? TGF β ? ?IL-10?? ?? ??? ????? ????? ????????? ? LineRegPCR ????? ??????? ?????? ???? ??-???. ????????? ????? ??-??? ?? ??-??? ??? ??????? ????? ????????? ?? ???-????? LinReg PCR ??????? ????.

机译：?????? ？ ???：?????? ????克隆??? ??????? ??实时PCR ???-????? ??? ????????? ??-??? ?????? ?? ??????? ????????? ??????? ?????? ???? ???? ???。 ??? ??? ?????? ?????? PCR ?????? ??????? ??? ?? ??????? ?? ?? ??? ??? ?? ?????? ????????? ？ ???-????????????? LinRegPCR ?????? ????????? ???? ???? ?????? ?????? PCB ??-????。??? ?????：?? ??? ?????? ?? ?? ???-???? ？ ???????脱氧核糖核酸？ ???? cDNA ?????? ？QRT-PCR ????? ??。??? ?????? PCR效率?? ?? ??? ??? ?????? ????????? ？ ?????? ?? ??????? ?? ???-????? LinReg PCB ????? ???? ？ ?? ?????? ??? ?? ??? ?????? ?? ?? ?????? ????。??????-??：????? ?????? PCR ?????? ??? ???? ?? ?? GAPDH？ TGFβ？ IL-10 ?? ??? ?????? ????????? ??-????? 99/1？ 81/1？ 87/1？ ?????? ?? ??? ??????? ?? ???-????? ??????-?? LinReg PCR ?????? ??? ?? ?? ??-????? 98/1？ 82/1？82/1 ??-??? ???。?????-????：????? ??? ?????? ???? ??-??? ?? ?? ?????? ????-??? ???? ?? ?????? cDNA ????? PCR ?????? ?????? ???? ???? ?? ?? GAPDH？ TGFβ？ IL-10 ?? ??? ?????? ?????? ????????? ？ LineRegPCR ????? ??????? ?????? ???? ??-???。 ????????? ?????? ??-??? ?? ??-??? ??? ??????? ?????? ????????? ?? ???-????? LinReg PCB ??????? ????。

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《Jundishapur Scientific Medical Journal》 |2012年第1期|共11页
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中图分类基础医学;
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Real-Time PCRPCR Efficiency??????????????????LinReg PCR;

机译：实时PCR的PCR效率LinReg PCR;

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????? ????? ?????? ??? Real-Time PCR ?? ??????? ?? ?? ?????? ??? ????? ????????? ? ??????? ???

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