Effect of acupuncture on the genetic expression of myocardial endothelin-1 and atrial natriuretic peptide in rats with stress-induced prehypertension

摘要

Objective To explore the protective effect of acupuncture against myocardial injury in rats with stress-induced prehypertension (SIPH) by observing the genetic expression of myocardial endothelin-1 (ET-1) and atrial natriuretic peptide (ANP). Methods Thirty-six Wistar rats were randomized into three groups: the control group, model group, and model?+?acupuncture (AP) group (n?=?12 rats per group). During the 11-day modeling period, the model group and the model?+?AP group experienced plantar electric stimulation in combination with noise exposure, and daily acupuncture intervention was applied simultaneously in the model?+?AP group; the control group did not experience modeling or acupuncture. Systolic pressure (SP) was measured the day before modeling began, and on the 3rd, 5th, 7th, 9th, and 11th day after modeling initiation. On the 12th day, histopathological observation of the left ventricle was made with hematoxylin-eosin staining and quantitative genetic expression of myocardial ET-1, and ANP was tested by quantitative real-time polymerase chain reaction (PCR). Results SP was higher in the model group than the control group on the 3 rd , 5 th , 7 th , 9 th , and 11 th days (all P ?