Detection of Avian Influenza, Newcastle Disease and Infectious Bronchitis Viruses in Domestic and Captive Migratory Wild Birds Using Nested Polymerase Chain Reaction, Yobe State Nigeria

摘要

Yobe State is located in Nigeria; it lies between latitudes 11°56? and 13°20?E and longitudes 9°38? and 12°25?N and shares boundary with Niger Republic. The state is known for rearing of livestock and local poultry production. Viruses are known to cause most of the important respiratory diseases of poultry, these diseases results in morbidity, mortality and decline in production. We conducted a survey to detect Avian Influenza (AI), Newcastle Disease (ND) and Infectious Bronchitis (IB) viruses in domestic and captive migratory wild birds using Nested Polymerase Chain Reaction (nPCR), We sampled 159 domestic and 34 wild migratory birds from poultry slaughter slabs located in four Local Governments Areas of Yobe state. Tissue samples were collected processed and tested using nPCR. The prevalence of ND virus was 30.8% and of IB virus was 2.5% in domestic birds. The prevalence of ND and IB viruses was highest (33.1% and 2.9% respectively) among local chickens when compared to other domestic birds. ND, IB, and AI viruses were not detected in wild migratory birds. Some of these viruses are zoonotic and of economic importance in poultry production, an effective control and prevention strategy should be established.