Effect of phospholipase A2 inhibitors during infection caused by Leishmania (Leishmania) amazonensis

摘要

Background Lipid metabolites play an important role in parasite differentiation and virulence. Studies have revealed that Leishmania sp. uses prostaglandins to evade innate barriers, thus enabling the parasites to survive inside immune cells. Despite the role of the enzyme Phospholipase Asub2/sub (PLAsub2/sub) in prostaglandins production, few studies have investigated the role of parasite PLAsub2/sub during the interaction between L. (L.) amazonensis and the host (in vitro and in vivo) immune cells. Methods In the present work, the leishmanicidal effect of PLAsub2/sub inhibitors, methyl arachidonyl fluorophosphonate (MAFP), bromoenol lactone (BEL) and aristolochic acid (AA) were investigated in vitro (promastigote and intracellular amastigote forms of L. (L.) amazonensis ) and during in vivo infection using BALB/c mice. Results The aforementioned inhibitors were deleterious to promastigote and amastigote forms of the L. (L.) amazonensis and were non-toxic to peritoneal macrophages from BALB/c mice. L. (L.) amazonensis -infected BALB/c mice treated with the inhibitor BEL presented decreased lesion size and skin parasitism; however, BEL treatment induced hepatotoxicity in BALB/c mice. Conclusions Results presented herein suggested that PLAsub2/sub inhibitors altered L. (L.) amazonensis viability. In spite of liver toxicity, treatment with BEL was the most selective compound in vitro, as well in vivo, resulting in lower skin parasitism in the infected mice. These findings corroborate the role of PLAsub2/sub in parasite virulence and maintenance in vertebrate hosts, and suggest that molecules structurally related to BEL should be considered when planning compounds against Leishmania sp.