Amperometric Biosensor for Hydrogen Peroxide Determination Based on Black Goat Liver-Tissue and Ferrocene Mediation

摘要

The response characteristics of the bioelectrode developed by the co-immobilization of black goat liver tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide were evaluated. In the range of electrode potential examined (-0.3~+0.0 V vs. Ag/AgCl), the response time was relatively short (t95%=12 s) and it responded in the wide range of pH. The detection limit was 2.25?0-6 M (S/N=3) and a relative standard deviation of the measurements which were repeated 15 times using 1.0?0-2 M hydrogen peroxide was 1.87%. The bioelectrode sensitivity decreased to 50% of the original value in 19 days of continuous use.