The effect of different speeds of centrifugation on platelet-rich plasma preparation

摘要

ercial products. However, there is no standardized method for PRP preparation. Objective To evaluate the effect of different speeds of centrifugation on PRP preparation. Patients and methods A volume of 10 ml of whole venous blood was collected from 20 healthy volunteers (10 female and 10 male), aged 20a??40 years. These samples were divided into two tubes and left to precipitate (sample 1) and the platelet concentration was measured in the supernatant. The sample was then resuspended and the first tube was centrifuged at low speeds of 200, 300, and 500 rpm, and the second tube was centrifuged at 700, 900, 1100, and 3000 rpm (samples 2a??8, respectively) and the platelet concentration was measured and compared in the obtained PRP. Platelet concentration was measured in the two PRP samples obtained from two more volunteers (both male) using the commercial Regen kit and compared with those obtained by means of the laboratory method from samples obtained from the same volunteers. Results The platelet concentration was the highest in the PRP obtained in samples 1 and 2, followed by that obtained in samples subjected to lower speeds of centrifugation (300 and 500 rpm), and it was significantly higher compared with that obtained using a ready-to-use kit. Moreover, the white blood cell concentration was higher in the PRP obtained using laboratory method compared with that obtained using the kit method. Conclusion Laboratory preparation of PRP, especially that obtained without centrifugation, or using lower centrifugation speed, yields higher platelet concentration. Centrifugation at low speed is preferable as it saves time, avoids sample contamination, and preserves platelets....