Preparation of blood platelet lysate for culture of animal cells, involves concentrating platelet-rich plasma, adding water and then calcium for forming coagel to the plasma, centrifuging the coagel, and filtering the blood platelet lysate

摘要

A blood platelet lysate derived from platelet-rich plasma from whole blood of animals, to which whole blood a citrate has been added to prevent coagulation, is prepared by concentrating platelet-rich plasma by ultra-filtration; adding water to the plasma for lysis of the platelets; adding calcium (Ca 2+) to the plasma for forming coagel from other components than lysed platelets; centrifuging the coagel comprising lysed platelets and a coagulate; and filtering the blood platelet lysate. The plasma, after addition of calcium, is allowed to stand at 37[deg]C (18-25[deg]C) maximum for 3-8 (5) hours.