Kinetics of the aromatic l-amino acid oxidase from dead bovine spermatozoa and the effect of catalase on fertility of diluted bovine semen stored at 5°C and ambient temperatures

摘要

Summary. At limiting velocity 50 × 106 dead spermatozoa consumed 13·6 μl O2/h and degraded 6·1 × 10?7 mol l-phenylalanine/h. Substrate concentration in egg yolk was equivalent to 6·1 × 10?3 m-l-phenylalanine. At a substrate concentration of 3 × 10?2 m-l-phenylalanine, oxidase activity was significantly affected by oxygen tension (r = 0·947, P < 0·01) and temperature (r = 0·995, P < 0·01). Non-return rates were not affected when semen was stored at 5°C in 20% egg yolk diluents with catalase but were significantly increased with semen stored at ambient temperatures of 15–23°C.