The activity of glyceraldehyde 3-phosphate dehydrogenase in spermatozoa from different regions of the epididymis in laboratory rodents treated with α-chlorohydrin or 6-chloro-deoxyglucose

摘要

Summary. The activity of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (mUnits/106 spermatozoa: mean ± s.e.m., N = 12) in spermatozoa from the rat epididymis declined from 22·0 ± 1·4 in the caput to 14·1 ± 1·3 in the corpus region but there was no further decrease in the cauda region. In hamsters (N = 4), GAPDH activity in spermatozoa declined from 24·8 ± 2·2 in the caput to 15·2 ± 1 ·2 in the distal cauda epididymidis with the greatest decrease between the corpus and proximal cauda regions. In guinea-pigs (N = 4) GAPDH activity in spermatozoa increased from 11·4 ± 0·79 in the caput to 18·0 ± 0·7 in the corpus and cauda regions of the epididymis. The activity of GAPDH in spermatozoa therefore changes during maturation in a species dependent manner.