Studies on Explant Regeneration and Protoplast Culture from Hypocotyl Segments of Jute, Corchorus capsularis L.

摘要

A repeatable protocol was developed for shoot regeneration from hypocotyl explants in jute ( C. capsularis ) that resulted in 25% regeneration using a combination of 6-BAP (7.5 mgl -1 ) and adenine hemisulfate (50 mgl -1 ). Rapid shoot proliferation was observed on medium containing NAA (0.1 mgl -1 ) and 6-BAP (1 mgl -1 ). The elongated shoots rooted on basal MS and could be successfully acclimatized, which later developed fertile flowers and viable seeds. After establishing regeneration protocol for jute from hypocotyl explants, the possibility of its response for protoplast isolation and culture was studied. The efficiency of isolation of protoplasts from hypocotyls was influenced by conditions of seedling growth, incubation time in enzyme solution, constitution of the enzyme mixture, osmoticum and purification methods. Isolated non-vacuolated protoplasts when cultured in modified Kao’s medium with 7.2 gl -l sucrose produced microcalli in about four weeks. However, these microcalli did not develop further.