Novel Biotechnological Products from Natural Sources: Nutri/ Pharmacogenomic Component

摘要

The presented study was targeted to establish a protocol for indirect organogenesis from leaf explants of Arnica montana, an important medicinal plant for pharmaceutical and cosmetic use. For callus induction, two different types of explants (leaf and petiole segments) from three-months-old in vitro plants were cultivated on Murashige and Skoog medium (MS) supplemented with 0.1 mg/l 2,4-D (2,4-dichlorophenoxyacetic acid). The efficiency of callus formation from leaf explants was 90%. Our results showed that shoot regeneration from leaf-derived callus was possible in MS medium supplemented with 1 mg/l BA (N°-benzyladenine) and 0.1 mg/l 2,4-D. However, the intensity of shoot regeneration was low (0.86 shoots/explant). The organogenic capabilities were demonstrated during two subcultivations on the same medium. The mean number of shoots per explant increased gradually during passages and reaches up to 2.1. For micropropagation, the regenerated plants were cultivated on MS media, supplemented with cytokinins: BA, Kinetin, Zeatin and 2-iP (6-y, y-dimethylallylamino purine) and auxin IAA (indole-3-acetic acid). Rapid proliferation of shoots (16.3 shoots/explant) was achieved onto MS medium supplemented with 1.0 mg/l BA and 0.1 mg/l IAA. All the micro-shoots produced normal roots on “2 MS medium containing 0.5 mg/l IBA (indole butyric acid) in four weeks of culture.