首页> 外文期刊>Journal of Oral Microbiology >Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples
【24h】

Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples

机译:未刺激和刺激唾液样品中细菌谱的比较分析

获取原文
       

摘要

Background and objective The microbial profiles of stimulated saliva samples have been shown to differentiate between patients with periodontitis, patients with dental caries, and orally healthy individuals. Saliva was stimulated to allow for easy and rapid collection; however, microbial composition may not reflect the more natural, unstimulated state. The purpose of this study was to validate whether stimulated saliva is an adequate surrogate for unstimulated saliva in determining salivary microbiomes. Design Unstimulated ( n =20) and stimulated ( n =20) saliva samples were collected from 20 orally and systemically healthy, non-smoking participants. Salivary bacterial profiles were analyzed by means of the Human Oral Microbe Identification using Next Generation Sequencing (HOMI NGS ), and statistical analysis was performed using Mann–Whitney test with Benjamini–Hochberg's correction for multiple comparison, cluster analysis, principal component analysis, and correspondence analysis. Results From a total of 40 saliva samples, 496 probe targets were identified with a mean number of targets per sample of 203 (range: 146–303), and a mean number of probe targets of 206 and 200 in unstimulated and stimulated saliva samples, respectively ( p =0.62). Based on all statistical methods used for this study, the microbial profiles of unstimulated and stimulated saliva samples collected from the same person were not statistically significantly different. Conclusions Analysis of bacterial salivary profiles in unstimulated and stimulated saliva samples collected from the same individual showed comparable results. Thus, the results verify that stimulated saliva is an adequate surrogate of unstimulated saliva for microbiome-related studies.
机译:背景和目的刺激唾液样品的微生物特征已显示出可区分牙周炎患者,龋齿患者和口腔健康患者。刺激唾液以便于快速收集。但是,微生物成分可能无法反映出更自然,更不受刺激的状态。这项研究的目的是验证刺激唾液是否是确定唾液微生物组学中未刺激唾液的适当替代品。设计从20名口服和全身健康的非吸烟参与者中收集未刺激(n = 20)和刺激(n = 20)的唾液样本。唾液细菌谱通过下一代测序(HOMI NGS)通过人类口腔微生物鉴定进行分析,并使用Mann-Whitney检验与Benjamini-Hochberg校正进行多重比较,聚类分析,主成分分析和对应性进行统计分析分析。结果从总共40个唾液样本中,鉴定出496个探针目标,每个样本的平均目标数目为203(范围:146–303),在未刺激和刺激的唾液样本中,平均探针目标数目为206和200,分别为(p = 0.62)。根据用于本研究的所有统计方法,从同一人收集的未刺激和刺激唾液样品的微生物谱在统计学上无显着差异。结论从同一个人收集的未刺激和刺激唾液样品中细菌唾液谱的分析显示出可比的结果。因此,结果证实了刺激的唾液是微生物组相关研究中未刺激唾液的适当替代品。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号