In vitro antibacterial effect of carbamide peroxide on oral biofilm

摘要

Thisstudycomparedtheeffectsofcarbamideperoxide(CP)andchlorhexidine(CHX)onoralbiofilminvitro.Collagen-coatedhydroxyapatitediscswereinoculatedwithsubgingivalplaque.After3weeks,theemergentbiofilmsweresubjectedto1-,3-,and10-minexposuresofa1%CHXgel,a5%CPgelandrinse,anda10%CPgelandrinse.Subsequently,thebiofilmswerestainedusingatwo-colourfluorescentdyekitforconfocallaserscanningmicroscopy,andthevolumeratioofdeadbacteriatoallbacteriawasanalysed.Comparedtoanon-treatedgelcontrol,theactiveagentskilledbacteriaonallthediscs,withhigherconcentrationandlongerexposuretimeskillingmorebacteria.Therinseformdisruptedthebiofilmquickerthanthegelform.Overall,10%CPshowedmoredisruptionofbiofilmandagreaterproportionofkilledbacteriathan1%CHX(pamp;0.05).Keywords:antibacterial;biofilm;carbamideperoxide;confocallaserscanningmicroscopy(Published:12June2013)Citation:JournalofOralMicrobiology2013,5:20392-http://dx.doi.org/10.3402/jom.v5i0.20392