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In vitro antibacterial effect of carbamide peroxide on oral biofilm

机译:过氧化脲对口腔生物膜的体外抗菌作用

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Thisstudycomparedtheeffectsofcarbamideperoxide(CP)andchlorhexidine(CHX)onoralbiofilminvitro.Collagen-coatedhydroxyapatitediscswereinoculatedwithsubgingivalplaque.After3weeks,theemergentbiofilmsweresubjectedto1-,3-,and10-minexposuresofa1%CHXgel,a5%CPgelandrinse,anda10%CPgelandrinse.Subsequently,thebiofilmswerestainedusingatwo-colourfluorescentdyekitforconfocallaserscanningmicroscopy,andthevolumeratioofdeadbacteriatoallbacteriawasanalysed.Comparedtoanon-treatedgelcontrol,theactiveagentskilledbacteriaonallthediscs,withhigherconcentrationandlongerexposuretimeskillingmorebacteria.Therinseformdisruptedthebiofilmquickerthanthegelform.Overall,10%CPshowedmoredisruptionofbiofilmandagreaterproportionofkilledbacteriathan1%CHX(pamp;0.05).Keywords:antibacterial;biofilm;carbamideperoxide;confocallaserscanningmicroscopy(Published:12June2013)Citation:JournalofOralMicrobiology2013,5:20392-http://dx.doi.org/10.3402/jom.v5i0.20392
机译:Thisstudycomparedtheeffectsofcarbamideperoxide(CP)andchlorhexidine(CHX)onoralbiofilminvitro.Collagen-coatedhydroxyapatitediscswereinoculatedwithsubgingivalplaque.After3weeks,theemergentbiofilmsweresubjectedto1-,3-,和10-minexposuresofa1%CHXgel,A5%CPgelandrinse,anda10%CPgelandrinse.Subsequently,thebiofilmswerestainedusingatwo-colourfluorescentdyekitforconfocallaserscanningmicroscopy,andthevolumeratioofdeadbacteriatoallbacteriawasanalysed.Comparedtoanon-treatedgelcontrol,theactiveagentskilledbacteriaonallthediscs,总体而言,10%CP显示生物膜的破坏程度更大,杀菌细菌的比例大于1%CHX(p <0.05)。 /10.3402/jom.v5i0.20392

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