The protective effect of hydro-alcoholic extract of mangrove ( Avicennia marina L.) leaves on kidney injury induced by carbon tetrachloride in male rats

摘要

Background: Materials can cause liver and kidney damage which carbon tetrachloride is one of these substances. Medicinal plants and their essential oils and extracts have been used to a large extent as drugs to better control and management of kidney diseases. Objectives: The aim of this study was to investigate the effect of hydro-alcoholic extract of Avicennia marina leaves in the treatment of renal toxicity induced by carbon tetrachloride. Methods: Forty-two male rats were randomly divided into 6 groups (n = 7): control (taking normal saline, 0.5 ml/day, intraperitoneally; i.p.), sham (taking olive oil, 0.5 ml/day, i.p., single dose), injury induced by carbon tetrachloride (CCl4) 1:1 with olive oil, 0.5 ml single dose, i.p.), treated groups 1, 2 and 3: by carbon tetrachloride 1:1 with olive oil, 0.5 ml single dose and 200 mg/kg, 400 mg/kg or 800 mg/kg Avicennia marina extract (AME)/ day for 96 hours, i.p.). By direct blood sampling from the heart, the plasma concentrations of lactate dehydrogenase, blood urea nitrogen (BUN), creatinine and liver enzymes including aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were measured. Kidney sections were prepared from all groups and the histological examinations were performed. The results were analyzed using one-way analysis of variance (ANOVA). Results: The results indicated the significant (P Conclusions: Carbon tetrachloride has kidney, liver and cardiac toxicities and mangrove extract is able to inhibit the toxicities of carbon tetrachloride