Ischemia Modified Albumin Levels in Spinal Cord Ischemia/Reperfusion Injury: an Experimental Study

摘要

Background :Spinal cord ischemia/reperfusion (I/R) injury involves evident ischemia-mediated oxidative stress. Ischemia-modified albumin (IMA) has emerged as a novel and sensitive ischemia and oxidative stress biomarker, although it has not been previously investigated in ischemic spinal cord injury (SCI). This study was therefore intended to investigate serum IMA levels in a rabbit model of experimentally induced ischemic SCI. Method :Spinal cord ischemia was induced in 53 mature, male New Zealand rabbits weighing 2.5-3 kg using the aortic occlusion model. Animals were divided into 10 groups (sham, ischemia and different duration I/R groups and their respective controls). IMA, tissue and blood malondialdehyde (MDA), tissue and blood myeloperoxidase (MPO) activity levels and histopathological damage scores were then compared. Results :Ischemia-modified albumin levels rose rapidly when ischemia was induced to the spinal cord. They then declined to levels similar to those of the control groups when reperfusion was established. Serum IMA levels exhibited a tendency to rise over time in both the control and I/R groups compared to the sham group. We observed no correlation between IMA levels and blood and tissue MDA and MPO levels (p>0.05 for all correlations). We determined only a low correlation between histopathological score and serum IMA and MDA levels. Conclusions :The results from this pioneering study identified a high level of IMA in spinal cord I/R injury, indicating that IMA is a potentially valuable diagnostic or predictive technique for ischemic SCI and its complications.