Medicinal plants were evaluated for their Xanthine Oxidase (XO) inhibitory potential. Their aqueous extracts, prepared from used parts, were tested in vitro, at 100 ?g/mL concentration, for their inhibition potencies expressed as % inhibition of XO activity. Two of the test plants were found inhibition% activity of xanthine oxidase. Namely Zingiber officinat (81.56±3.76) , Momdica charantia (96.5±2.17).Total Phenolic of Momdica charantia (80.83±0.30) was more than Zingiber officinat (62.18±0.65).Fraction of Momdica charantia (coumarin) was achieved the highest activity (inhibition activity of xanthine oxidase 97%) based on analysis HPLC. Screening of xanthine oxidase activity by coumarin in terms of kinetic parameter revealed uncompetitive mode of inhibition where Km 25Mm-1,Vmax 0.38 Mm min-1.Data showed that coumarin (0.5 mg/kg) treatment cause significant reduction in the serum uric acid level of hyperuricemia in normal mice. These finding suggest that Momdica charantia extract possess prominent medicinal properties and can be exploited as natural drug to treat diseases associated with free radical formation , oxidative stress and xanthine oxidase activity. Keywords : Gout , Allpurinol , Xanthine Oxidase , Momdica charantia
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机译：评价药用植物的黄嘌呤氧化酶（XO）抑制潜力。他们从用过的部分制备的水提物在100μg/ mL的浓度下进行了体外测试，其抑制能力表示为对XO活性的抑制％。发现两个测试植物抑制了黄嘌呤氧化酶的％活性。苦瓜（81.56±3.76），苦瓜（96.5±2.17），苦瓜总酚（80.83±0.30）超过苦瓜（62.18±0.65），苦瓜的香豆素活性最高（根据分析型HPLC）（黄嘌呤氧化酶的抑制活性为97％）。香豆素对黄嘌呤氧化酶活性的动力学参数筛选显示出非竞争性抑制模式，其中Km 25Mm-1，Vmax 0.38 Mm min-1。数据表明，香豆素（0.5 mg / kg）处理可导致血清尿酸水平显着降低正常小鼠的高尿酸血症。这些发现表明苦瓜提取物具有突出的药用特性，可以用作治疗与自由基形成，氧化应激和黄嘌呤氧化酶活性有关的疾病的天然药物。关键词：痛风，嘌呤醇，黄嘌呤氧化酶，苦瓜。
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