Parthenolide Cytotoxic Effect on GC-2SPD Cancer Cell Line

摘要

The purpose of this experiment is to test the effect of the drug Parthenolide on cancer cell line GC-2SPD (2196) in vitro. This will be done by testing dose range of the drug at different two temperature; 32C, and 39C. The cells were grown at in a very similar environment as they were inside the body and they were conditioned to grow in spheroid architecture to resemble their structure as solid tumor in the tissue. Growth material was needed to maintain viable cells and routine subculture was performed as needed. Cells were drugged with different concentrations of Parthenolide and assayed, and then they were let to recover for some time and then assayed again to actually asses the ratio of viable cells to dead cells. Although there were some unintended mistakes, the experiment was successful and yielded good results. The cells showed more sensitivity at 39C as photos and curves showed.