Antimicrobial activity of essential oil extract of Ocimum basilicum L. leaves on a variety of pathogenic bacteria

摘要

As in the recent years the usage of the herbal materials has been increased, it seems necessary to study the antibacterial effects of them. The basil herb, which is easily cultured worldwide, may be a potentially good candidate to be used as a plant with antibacterial activity. The essential oil was distilled using a Clevenger-type apparatus and extracted from plant leaves. The antibacterial properties of basil essential oil was studied on the standard gram-negative bacteria including?Escherichia coli,?Pseudomonas aeruginosa, and gram-positive ones including?Bacillus cereus,?Staphylococcus aureus, then agar disk diffusion, minimal inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were detected. The results of agar disk diffusion tests showed the inhibition zones as follow:?S. aureus?29.20-30.56 mm,?B. cereus?10.66-16.11 mm,?E. coli?17.48-23.58 mm and for?P. aeruginosa?the maximum inhibition zones were seen. The results of this study showed the presence of bacteriostatic effects of basil essential oil on all the test bacteria. The MICs for gram-positive bacteria were as:?B. cereus?ranging 36-18 μg/mL,?S. aureus?18 μg/mL, and for gram-negative bacteria of?E. coli?and?P. aeruginosa?were 18-9 μg/mL.