Shifting Immune Response and Cytokine Profiles After Porphyromonas gingivalis Lipopolysacaride Exposure

摘要

There is a tendency that child who doesn’t have any family history of atopic disease will easilysuffer asthma or hay fever when they lived with people who have. It is believed that hygienehypothesis should be revisited again, while some research suggest that periodontal pathogensPorphyromonas gingivalis (Pg) play significant role in the presence ofsome atopic diseases.We compared level of cytokine profile for type I hypersensitivity markers in wistar rats after exposure of lipopolysaccharide (LPS) of Porphyromonas gingivalis (Pg).An experimental study with pretest-posttest controlled group design were done in 1 January-10December 2016. We used 3 groups of wistar rats, given exposure in the various dose of LPSPgand 1 group of wistar rats given placebo. 11 days later, we measured level of cytokine profilesIFN-γ, IL-2, IL-4, IL-5, IgE and IgG4 by ELISA.There is a shifting from a non-atopy rat became an atopy rat. After 11 days, there is nosignificant different in Th1 activity (represented by IFN-γ and IL-2), whereas there is a significantdifferent in Th2 activity(represented by IL-4 and IL-5). Exposure of LPS Pg also stimulatesincreasing of IgE and IgG4.There is a significant shifting immune response and cytokine profiles after LPS Pg exposures.Nevertheless, manifestation of atopic diseases and allergic asthma are not clearly appears due tothe increasing level of IgG4 as counter-response to increasing of IgE.