Effects of allogeneic PRP combined with ADSC on wound healing and serum HA and VEGF levels in mice

摘要

Objective: Elet-rich plasma (PRP) combined with adipose-derived mesenchymal stem cells(ADSC) on skin wound healing and serum hyaluronic acid (HA) and vascular endothelialgrowth factor (VEGF) levels in mice. Methods: Forty-five mice were randomly divided intothree groups (n=15), which were control, ADSC, and ADSC+PRP. The wound injury modelwas established in all three groups. The control group was injected subcutaneously withnormal saline, the ADSC group was injected subcutaneously with allogeneic PRP, and theADSC+PRP was injected subcutaneously with ADSC and PRP. Wound healing was observedat 3 d, 5 d, and 7 d after modeling, and histomorphological features were observed by HEstaining. Collagen I and Collagen III levels were detected using Western blot. The levels ofserum HA and VEGF in each group were detected and compared. Results: The wound healingrate of ADSC group and ADSC+PRP group was significantly higher than that of control groupat 3 d, 5 d and 7 d after modeling (P0.01). The wound healing rate of the ADSC+PRP groupwas significantly higher than that of the ADSC group (P0.01). The inflammatory response ofthe control group and the ADSC group was significant at 3 and 5 d after modeling, and therewas a small amount of granulation tissue in the ADSC group. The ADSC+PRP group had amilder inflammatory response and more granulation tissue; After 7 d, the ADSC+PRP grouphad more neovascularization and higher re-epithelialization rate. The control group and theADSC group had poorer epithelialization and fewer new blood vessels. The expression levelsof Collagen I and Collagen III in ADSC group and ADSC+PRP group were significantlyhigher than those in control group at 3 d, 5 d and 7 d after modeling (P0.01). Collagen I andCollagen III in the ADSC+PRP group were significantly higher than those in the ADSC group(P0.01). Serum HA and VEGF in ADSC group and ADSC+PRP group were significantlyhigher than those in control group at 3 d, 5 d and 7 d after modeling (P0.01). Serum HA andVEGF were significantly higher in the ADSC+PRP group than in the ADSC group (P0.01).Conclusion: Allogeneic PRP combined with ADSC can promote the regeneration and healingof skin wounds in mice. The mechanism may be related to the up-regulation of the expressionof vascular endothelial growth factor and HA and the promotion of the formation of bloodvessels and granulation tissue.