PRODUCTION, CHARACTERIZATION AND ANTIMICROBIALACTIVITY OF SILVER NANOPARTICLES PRODUCEDBY PEDIOCOCCUS ACIDILACTICI

摘要

In this study antimicrobial potential of silver nanoparticles (AgNPs) synthesised by Pediococcus acidilactici against non bacteriocin producing Bacillus subtilis and its antimicrobial potential on human pathogens were evaluated. Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) confirmed that both AgNPs preparations were consistent in their spherical shape, with average diameters of 16 nm and 12 nm derived from culture free supernatants of P. acidilactici and B. subtilis, respectively. The presence of functional groups over the surface of nanoparticles was confirmed by Fourier transform infrared spectroscopy (FTIR) and Energy dispersive X-ray (EDX) analysis. Minimum inhibitory concentration (MIC) of AgNPs displayed no significant difference (p?0.05) in antibacterial activity. Antifungal activities of AgNPs preparations were statistically significant (p?0.05) in the case of C. albicans and C. krusei. These results highlights that sunlight irradiation, a relatively unexplored primary energy source was able to photo-catalyse nanoparticle formation completely and AgNPs synthesised by Pediococcus acidilactici and B. subtilis extracts with potential for application as broad-spectrum antimicrobial agents.