Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice’s CD4+CD25+ and B220+ Lymphocyte Cells

摘要

This experiment was aimed to determine the bioactivity of ethanolic extract of propolis (EEP) against the changes in the quantity of CD4~(+)CD25~(+ )and B220~(+ )lymphocytes and determine the optimal dose of EEP to increase the number of CD4~(+)CD25~(+) and B220~(+) cells in Balb/c mice. Balb/c mice were divided into four treatment groups: control treatment, treatments of EEP at a dose of 50 mg.kg~(-1), 100 mg.kg~(-1), and 200 mg.kg~(-1 )body weight of mice. All mice were dissected after two weeks post treatment. Profiles of lymphocytes from the spleen expressing CD4~(+)CD25~(+) and B220~(+) cells were analyzed by flowcytometry using CellQuest software. Data was analyzed by Kruskall Wallis and Mann Whitney statistical test with P<0.05 using SPSS 16.0 for Windows. The results showed that the treatment of dose of 50 mg.kg~(-1 )of EEP can increase relative number of CD4~(+)CD25~(+) cells significantly, but those cells decrease significantly when we apply the dose of 100 and 200 mg.kg~(-1). The relative number of B220~(+) cells increase in the dose of 50 and 200 mg.kg~(-1 )and decrease in the dose of 100 mg.kg~(-1) compared to the control. This experiment suggest that EEP has bioactivity to modulate the quantity of CD4~(+)CD25~(+) and B220~(+ )in dependent manner.Keywords: B220~(+), CD4~(+)CD25~(+), lymphocyte, propolis.